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The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:
• Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
• Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
• Simple—easy to use, with a single reagent kit for all cell types
• Versatile—open system allows electroporation parameters to be optimized freely
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The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination
1 There are twelve tubes in two basket assembly.
2 Each test station is driven independently. Auto stop at timing point.
3 The bath liquid can achieve an even temperature.
4 A MPU is used to automatically control the temperature, time and frequency of nacelle back and forth.
5 Timing at will. The preset and real time data can be displayed alternately in time.
6 Automation: auto-test, auto-diagnose, auto-alarm.
Laminar Flow Cabinet – is used only for sample protection. Laminar Flow Cabinet is a work bench or similar enclosure, which creates a particle-free working environment by taking air through a filtration system and exhausting it across a work surface in a laminar or unidirectional air stream.
Gentian violet slightly stains the nuclei of the leucocytes. The blood specimen is diluted 1:20 in a WBC pipette with the diluting fluid and the cells are counted under low power of the microscope by using a counting chamber. The number of cells in undiluted blood is reported per cumm (µl) of whole blood.
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