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The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:
• Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
• Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
• Simple—easy to use, with a single reagent kit for all cell types
• Versatile—open system allows electroporation parameters to be optimized freely
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1 There are twelve tubes in two basket assembly.
2 Each test station is driven independently. Auto stop at timing point.
3 The bath liquid can achieve an even temperature.
4 A MPU is used to automatically control the temperature, time and frequency of nacelle back and forth.
5 Timing at will. The preset and real time data can be displayed alternately in time.
6 Automation: auto-test, auto-diagnose, auto-alarm.
Stand: Microscope stand configuration AFL
Objective: Objective A 4 / 0,10, Objective A 10 / 0,25, Objective SPL 20 / 0,35 (LD), SPL 40 / 0,60 (LD) in correction mount
condenser: Brightfield condenser NA 0,25 for attachment to the illumination unit
Illumination: HBO 100
Lamphouse: HBO 100 with collector, mirror, lamp mount and heat protection filter
HBO LAMP: Mercury high pressure lamp 100 W
Power supply: HBO 100 including mains cable
Filter slider: Filter slider for up to 2 filtersets
FITC Filter: Filter set D for immunofluorescence (FITC) blue excitation
Set of accessories: Set of accessories WS AFL, operating manual, dust cover
Gentian violet slightly stains the nuclei of the leucocytes. The blood specimen is diluted 1:20 in a WBC pipette with the diluting fluid and the cells are counted under low power of the microscope by using a counting chamber. The number of cells in undiluted blood is reported per cumm (µl) of whole blood.
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