Transfection system

The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:

• Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
• Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
• Simple—easy to use, with a single reagent kit for all cell types
• Versatile—open system allows electroporation parameters to be optimized freely