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WBC pipette, diluting fluid, compound microscope, sterile lancet, watch glass, rectified spirit, cotton. WBC Pipette this is a bulb pipette having a long stem with a capillary bore and a pointed tip. The bulb contains a white bead inside
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Laminar Flow Cabinet – is used only for sample protection. Laminar Flow Cabinet is a work bench or similar enclosure, which creates a particle-free working environment by taking air through a filtration system and exhausting it across a work surface in a laminar or unidirectional air stream.
Gentian violet slightly stains the nuclei of the leucocytes. The blood specimen is diluted 1:20 in a WBC pipette with the diluting fluid and the cells are counted under low power of the microscope by using a counting chamber. The number of cells in undiluted blood is reported per cumm (µl) of whole blood.
The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:
• Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
• Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
• Simple—easy to use, with a single reagent kit for all cell types
• Versatile—open system allows electroporation parameters to be optimized freely
Glass test tube, also known as culture tube and sample tube, is a common and widely used glassware, which has a finger-like length, open top and closed bottom. It can be made of soda-lime material, borosilicate glass or quartz glass. And borosilicate glass and quartz glass test tube can withstand high temperatures up to several hundred celsuis.
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